Protein Info for MPMX19_04034 in Azospirillum sp. SherDot2

Annotation: Multidrug export protein EmrB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 530 transmembrane" amino acids 26 to 50 (25 residues), see Phobius details amino acids 65 to 86 (22 residues), see Phobius details amino acids 94 to 113 (20 residues), see Phobius details amino acids 119 to 140 (22 residues), see Phobius details amino acids 152 to 173 (22 residues), see Phobius details amino acids 179 to 202 (24 residues), see Phobius details amino acids 222 to 241 (20 residues), see Phobius details amino acids 249 to 271 (23 residues), see Phobius details amino acids 291 to 312 (22 residues), see Phobius details amino acids 324 to 344 (21 residues), see Phobius details amino acids 356 to 373 (18 residues), see Phobius details amino acids 381 to 404 (24 residues), see Phobius details amino acids 501 to 525 (25 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 28 to 514 (487 residues), 434.3 bits, see alignment E=3.1e-134 PF00083: Sugar_tr" amino acids 29 to 206 (178 residues), 44.3 bits, see alignment E=1.9e-15 PF07690: MFS_1" amino acids 33 to 433 (401 residues), 212.6 bits, see alignment E=1.2e-66

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 71% identity to mno:Mnod_0313)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (530 amino acids)

>MPMX19_04034 Multidrug export protein EmrB (Azospirillum sp. SherDot2)
MSAAAEGADTGGFDASQSAAGGRNPWLIAVVVSIATFMEVLDTTIANVALRYISGTLAVS
ADEASWVVTSYLVANAIVLTASSFIAKRYGRKRFYLACLGLFTVSSLACGFAWNLESLLL
FRVLQGFAGGGMVPISQSILADSFPPAKRGQAFAIFGIAVVVAPVVGPTLGGWLSDNLSW
HWCFLINGPVGVMAFTLVSFLVQEPKSAIEERRRLKRQGIRMDVVGFLLVATFLGSLEVV
LDRGQTDDWFASGFIIAFAVICVASFCLMIPWEMTRENPILDLRMVATRQFGSCFLVMLA
TGAILIATTQFLPELLQQYYGFTATWAGLALSPGGMVTMVMMLVVGRLAGRIQPKYLIAT
GATIVALAMWDLTRLNPDSTFWFFAWSRIYIGIGLPLIFIPITTASYDGIPRDKTDQASA
LINVARNVGGSIGVSIAQNVLAFREQFHHSRLAEHVVPSNPAYQRTVEQATSYFSMAGGE
AGARERAVAYIGQQVQMQTAYWSYIDVFFALMLISASAVPLALILRTVKR