Protein Info for MMJJ_RS05390 in Methanococcus maripaludis JJ

Annotation: tRNA pseudouridine(13) synthase TruD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 PF01142: TruD" amino acids 2 to 384 (383 residues), 267.5 bits, see alignment E=1e-83 TIGR00094: tRNA pseudouridine synthase, TruD family" amino acids 2 to 385 (384 residues), 182.1 bits, see alignment E=1.2e-57

Best Hits

Swiss-Prot: 98% identical to TRUD1_METMP: Probable tRNA pseudouridine synthase D 1 (truD1) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K06176, tRNA pseudouridine synthase D [EC: 5.4.99.12] (inferred from 98% identity to mmp:MMP0014)

Predicted SEED Role

"tRNA pseudouridine 13 synthase (EC 4.2.1.-)" in subsystem tRNA processing (EC 4.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.-, 5.4.99.12

Use Curated BLAST to search for 4.2.1.- or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (389 amino acids)

>MMJJ_RS05390 tRNA pseudouridine(13) synthase TruD (Methanococcus maripaludis JJ)
MKFRQKHEDFIVTEILDYELNDYGNYFLYKLQKNGIENLKAISYLSKNFEVPTKEIGYCG
LKDRHAITTQYVSIPKEYGKLSLDEDNLKLEYVGTIEKPLKIGRLYGNRFEIIARAIDKD
EFLKIADNIRTLSSGAPNYYDDQRFGSVFNGKFIAKEILKGNYEEVVKILLTSYTKSEKK
QLKDLKRFIAKNWGNWDECLKYIDKKQIRSKMFRNMVKSLTYENDFKKAFKYVDNRLKEL
FISAYQSYLWNECLKEFLKEVIPKENRIYVDYSCGTFLFYENIDEELFVKLKEMDFPTIV
SDVEYTDSEKRIIKAILKKERIKISDFEKLDFGKLKYTKRLIISIPEDVNTGKFKSDELN
SKKYKIDLEFSLKKGSYATIILKRVFNIL