Protein Info for GFF5225 in Sphingobium sp. HT1-2

Annotation: Oleate hydratase (EC 4.2.1.53)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 transmembrane" amino acids 25 to 42 (18 residues), see Phobius details PF06100: MCRA" amino acids 23 to 520 (498 residues), 698.9 bits, see alignment E=2.1e-214

Best Hits

Swiss-Prot: 54% identical to OLHYD_MACCJ: Oleate hydratase (MCCL_0076) from Macrococcus caseolyticus (strain JCSC5402)

KEGG orthology group: K10254, myosin-crossreactive antigen (inferred from 89% identity to rpc:RPC_3601)

Predicted SEED Role

"Oleate hydratase (EC 4.2.1.53)" (EC 4.2.1.53)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.53

Use Curated BLAST to search for 4.2.1.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (588 amino acids)

>GFF5225 Oleate hydratase (EC 4.2.1.53) (Sphingobium sp. HT1-2)
MHYSSGNYEAFVRPRKPEGADRKTAWFVGSGLAGLAGAAFLIRDGGVAGEHITILEELHI
PGGALDGLDVPEKGFVIRGGREMEEHFECLWDLYRSIPSLEIEDASVLDEFYRLNKDDPN
FSLQRVTQNQGQDVPDKALLTLGDRARRDLISVFLATREEMENKRINEVFGDDFLRSNFW
LYWRTMFAFEEWHSALEMKLYLHRFIHHIGSLADFSSLKFNRYNQYESMVLPLVKWLQDR
GVRFRYGVEVTDVDFDIQPGRKQATRIARIENGVEGGVDLGADDLVFITIGSLTENSDNG
DHHTPARLNEGPAPAWDLWRRIAAKDPAFARPDVFGSHIPETKWASATITTLDARIPAYI
RKIAKRDPFSGTVVTGGIITVKDSSWLLSWTVSRQPHFRKQPKDQIVAWFYALFVDRPGD
YVKKPMQDCTGEEITQEWLYHLGVPVEDIPGLAATGAKTVPVMMPYITAFFMPRQAGDRP
DVVPEGAVNFAFVGQFAESKQRDCIFTTEYSVRTPMEAVYTLMKVERGVPEVFNSTYDIR
TLLAAIGPLRDGKGIDIPGPSFLRKLLMTKLEGTEIAQLLEEFHLLSR