Protein Info for GFF1442 in Xanthobacter sp. DMC5

Annotation: putative FAD-linked oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 PF01565: FAD_binding_4" amino acids 49 to 185 (137 residues), 147.3 bits, see alignment E=2.3e-47 PF02913: FAD-oxidase_C" amino acids 223 to 462 (240 residues), 213 bits, see alignment E=5.8e-67

Best Hits

Swiss-Prot: 54% identical to LDHD_MOUSE: Probable D-lactate dehydrogenase, mitochondrial (Ldhd) from Mus musculus

KEGG orthology group: K00102, D-lactate dehydrogenase (cytochrome) [EC: 1.1.2.4] (inferred from 85% identity to azc:AZC_1415)

Predicted SEED Role

"D-Lactate dehydrogenase, cytochrome c-dependent (EC 1.1.2.4)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.4

Use Curated BLAST to search for 1.1.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (474 amino acids)

>GFF1442 putative FAD-linked oxidoreductase (Xanthobacter sp. DMC5)
MSAHMPIAAHPMDGALARLAEIVGERATRAPGVLADHGRSEAYHPATPPDVVVYPETTAE
VQAIVRLCREMKLPVVAFGAGTSLEGNAAALHGGVCLDMGRMNAVLKVNAEDMDVTVQPG
ITRKQLNAQLRDTGLFFPIDPGADASIGGMTSTRASGTMAVRYGTMKDNVLALEVVLADG
RVIRTAQRARKSAAGYDLTRLFVGAEGTLGVITQVTLKLHPQPEAISSAVCAFPDLKAAV
DTAIAVIQSGVPVARVELLDAVMMRGMNLHAKLGLPEVPHLFFEFHGSKAYVAEQAETTE
ALAAEFGGEGFQWATTPEERSRLWHARDNTLYAGLALRPGAKAMITDVCVPISRLAECLV
ETAADIAQTGLVAPVVGHVGDGNFHLLVLIDPASTEELARAKNFCDRLARRAIALEGTCT
GEHGIGFGKMDFLPLELGDAVDVMRLVKGALDPDGILNPGKIFRPRDRQQGDAP