Protein Info for EX28DRAFT_0443 in Enterobacter asburiae PDN3

Annotation: Periplasmic protein involved in polysaccharide export

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF02563: Poly_export" amino acids 81 to 164 (84 residues), 86.5 bits, see alignment E=2.2e-28 PF22461: SLBB_2" amino acids 170 to 248 (79 residues), 84.6 bits, see alignment E=8.4e-28 amino acids 255 to 342 (88 residues), 86.5 bits, see alignment E=2.2e-28 PF10531: SLBB" amino acids 172 to 218 (47 residues), 30.7 bits, see alignment 4.4e-11 amino acids 255 to 306 (52 residues), 31.4 bits, see alignment 2.8e-11 PF18412: Wza_C" amino acids 345 to 374 (30 residues), 56.9 bits, see alignment (E = 2.7e-19)

Best Hits

Swiss-Prot: 76% identical to GFCE_ECO57: Putative polysaccharide export protein GfcE (gfcE) from Escherichia coli O157:H7

KEGG orthology group: K01991, polysaccharide export outer membrane protein (inferred from 87% identity to enc:ECL_03429)

MetaCyc: 61% identical to outer membrane polysaccharide export protein Wza (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Polysaccharide export lipoprotein Wza" in subsystem Colanic acid biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (379 amino acids)

>EX28DRAFT_0443 Periplasmic protein involved in polysaccharide export (Enterobacter asburiae PDN3)
MKSTTAFSLLFLTVSQLTGCVFSPGQNLSVSGKKVIAAEDVNYNLDKRVEIFPLTPGLIE
KLRPTVSKSQANPSLDEQIKNWEYRIGTGDILTVTVWDHPELTTPAGQYRSASDAGNWVN
ADGTLFYPYVGKLQVAGKTVSQVREEITARLDKVIESPQVDVSIAAFRSQKAYVTGEVEK
SGQQPITNIPLTVMDAVNAAGGLSADADWRNVVLTHDGKDSRLSLYTLMQHGDLTQNKLL
YPGDILFIPRNDALKVFVMGEVVKQSTLKMDRSGMTLAEALGNAGGLNQNMADATGIFVI
RQISKKGDTDKIANIYQLNAEDASAMVLGTEFQLEPYDIVYVTTAPLSRWNRVITQLVPT
ISGVHDLTETVRYIRSWPQ