Protein Info for EX28DRAFT_0410 in Enterobacter asburiae PDN3

Annotation: Glutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 transmembrane" amino acids 309 to 328 (20 residues), see Phobius details PF00120: Gln-synt_C" amino acids 148 to 458 (311 residues), 255.8 bits, see alignment E=3.2e-80

Best Hits

KEGG orthology group: None (inferred from 56% identity to esc:Entcl_1590)

Predicted SEED Role

"glutamine synthetase family protein" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>EX28DRAFT_0410 Glutamine synthetase (Enterobacter asburiae PDN3)
MNAFFLKSLRPYFNFPGLSLPGALNSGLWLDEKIDALQHNISVEVADYLERYPQTKHVDV
YLNDINGTMRGKRLSVESMLSLEKGCYFPLSVYSMDQKGKIAAPLHDEPDRLCVPVAGSL
RPSPQDPQHNAQILLTMKDSDNTPCPLEPRVILQNVLARFHQHGLFPVIAPEIEFYLTGH
DDRDPQNQGCFHMDTTTAHAALFDELEQLAHLQRIPMCGIVAEAESGQYELNLKHSHRVV
EVCDNVLALRRLTRFVAEKHGLQANFMAKPFSQLSGSGLHFHFSLNNSRGENVFASPVDA
LNSMMRLCIAGQLALMPASVAILAPGVNAFRRLRKNLTEPVFNSWGYNTRSAALRIPCSD
DHNRRIEYRLAGADANPYLVAATILTGMLYGLENTDDEDLPEPQHDRPELPLFQQEAIET
FARCQYLTDSLGDAFSEQWVACKLSELDWFERIVTREESQLA