Protein Info for BWI76_RS24825 in Klebsiella michiganensis M5al

Annotation: 2-hydroxy-3-oxopropionate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 PF02826: 2-Hacid_dh_C" amino acids 3 to 117 (115 residues), 31.4 bits, see alignment E=3.1e-11 TIGR01505: 2-hydroxy-3-oxopropionate reductase" amino acids 4 to 294 (291 residues), 510.8 bits, see alignment E=5.2e-158 PF03807: F420_oxidored" amino acids 4 to 95 (92 residues), 36.8 bits, see alignment E=1.2e-12 PF03446: NAD_binding_2" amino acids 4 to 163 (160 residues), 185.6 bits, see alignment E=1.7e-58 PF14833: NAD_binding_11" amino acids 166 to 283 (118 residues), 138.4 bits, see alignment E=3.8e-44

Best Hits

Swiss-Prot: 98% identical to GARR_ECOL6: 2-hydroxy-3-oxopropionate reductase (garR) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00042, 2-hydroxy-3-oxopropionate reductase [EC: 1.1.1.60] (inferred from 98% identity to eco:b3125)

MetaCyc: 98% identical to tartronate semialdehyde reductase (Escherichia coli K-12 substr. MG1655)
2-hydroxy-3-oxopropionate reductase. [EC: 1.1.1.60]

Predicted SEED Role

"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.60

Use Curated BLAST to search for 1.1.1.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BA75 at UniProt or InterPro

Protein Sequence (296 amino acids)

>BWI76_RS24825 2-hydroxy-3-oxopropionate reductase (Klebsiella michiganensis M5al)
MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIAEVIAAGAETAASAKAIAEQCD
VIITMLPNSPHVKEVALGEGGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGVDMLDA
PVSGGEPKAIDGTLSVMVGGDKAIFDKYYELLKAMAGSVVHTGEIGAGNVTKLANQVIVA
LNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR