Protein Info for BWI76_RS16785 in Klebsiella michiganensis M5al

Annotation: peptidase M20

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 TIGR01891: amidohydrolase" amino acids 12 to 368 (357 residues), 355.5 bits, see alignment E=1.8e-110 PF01546: Peptidase_M20" amino acids 69 to 380 (312 residues), 157.2 bits, see alignment E=5.6e-50 PF07687: M20_dimer" amino acids 181 to 274 (94 residues), 51.4 bits, see alignment E=9.7e-18

Best Hits

Swiss-Prot: 39% identical to ILL7_ORYSJ: IAA-amino acid hydrolase ILR1-like 7 (ILL7) from Oryza sativa subsp. japonica

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 85% identity to kva:Kvar_2183)

Predicted SEED Role

"Metal-dependent amidase/aminoacylase/carboxypeptidase"

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B568 at UniProt or InterPro

Protein Sequence (383 amino acids)

>BWI76_RS16785 peptidase M20 (Klebsiella michiganensis M5al)
MAVSSSLIAEAIRWRRDFHACPELGYQEQETSRRVAELLASFGLQVHRGLAGTGVVATLE
NGPGPVIGLRADMDALPIAEQSDVGYKSRNPGVMHACGHDGHSAMLLATAAHLAQTRRFR
GTVHFVFQPAEENLGGARKMVEEGLFERFPMDAIYALHNWPGMPLGQVAIGSGAMMASLD
AFEITLTGKSCHAAMPERGADPIVAAAQLIMALQTIPSRRLSPQESTVVSITQIAGGEAI
NVLPDKVVLRGTFRCLDNQVRARVRGLIESYVAAQPQVSEVEGAIAWYPGYPVTTNHPAE
AQKVREVATALLGESAVSWNGNPSMASEDFACMLEACPGAYFWIGADGETASKPLHNAGY
DFNDELLPHGVALWTALVEKLLA