Protein Info for BWI76_RS10820 in Klebsiella michiganensis M5al

Annotation: iron uptake system protein EfeO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF13473: Cupredoxin_1" amino acids 13 to 115 (103 residues), 86.8 bits, see alignment E=9.6e-29 PF09375: Peptidase_M75" amino acids 135 to 367 (233 residues), 148 bits, see alignment E=4.6e-47

Best Hits

Swiss-Prot: 88% identical to EFEO_ECO57: Iron uptake system component EfeO (efeO) from Escherichia coli O157:H7

KEGG orthology group: K07224, putative lipoprotein (inferred from 94% identity to kpu:KP1_2036)

MetaCyc: 88% identical to ferrous iron transport system protein EfeO (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Ferrous iron transport periplasmic protein EfeO, contains peptidase-M75 domain and (frequently) cupredoxin-like domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1Z7 at UniProt or InterPro

Protein Sequence (375 amino acids)

>BWI76_RS10820 iron uptake system protein EfeO (Klebsiella michiganensis M5al)
MMNHFRRNALQLTLAALFSSAFYAQAADIPQVKVTVTDKQCEPMNVTVKAGKTQFIIQNH
SQKALEWEILKGVMVVEERENIAPGFTQKLTANLQPGEYDMTCGLLTNPKGKLTVTGEAT
KDAAKADALLSLGEAITAYKAYVTAETAELVSGTKAFTDAVKAGDIEKAKALYAPTRQHY
ERIEPIAELFSDLDGSIDAREDDYEKKAEDPKFTGFHRLEKALFGDNSVKGMDNYADQLN
ADVLELQKRISELAFPPSKVVGGAAGLIEEVAASKISGEEDRYSHTDLWDFQANIDGAQK
IVDLLRPQLQKENSALLAKVDANFKKVDTILSKYRTKDGFETYDKLTDADRNALKGPITT
LAEDLAQLRGILGLD