Protein Info for ABZR87_RS01950 in Ralstonia sp. UNC404CL21Col

Annotation: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase
  • amino acids 10 to 488 (479 residues), 382.2 bits, E=1.9e-118
• PF21799: MurD-like_N
  • amino acids 10 to 90 (81 residues), 55.8 bits, E=7.1e-19
• PF08245: Mur_ligase_M
  • amino acids 127 to 257 (131 residues), 87.6 bits, E=1.7e-28
• PF02875: Mur_ligase_C
  • amino acids 355 to 468 (114 residues), 58.6 bits, E=1.8e-19

Best Hits

Swiss-Prot: 89% identical to MURD_RALSO: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 97% identity to rpf:Rpic12D_2726)

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Protein Sequence (499 amino acids)

>ABZR87_RS01950 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (Ralstonia sp. UNC404CL21Col)
MFGEFDAPVVLVLGLGESGLAMARWCARHGARVRVADTREAPANLPALRAHVPDAEFVSG
PFAVSLLDGVTLVAISPGLSPLDANVAALLSAAKAQSVPVWGEIELFARALAGLKAAQGY
APRVLAITGTNGKTTTTALTGALVERAGKTVGVAGNISPSALDKLSECVDAETLPDVWVL
ELSSFQLETTHTLDADAATVLNITQDHLDWHGTLEAYAAAKGRIFGANTVRVLNRQDAGV
MAFASKNGGDITFGIDEPGTPDALGLLRDGGMPWIVLAEADEEDLPKPTRRKKGDNAPAA
PVPVRLKRLMPADALRIRGLHNATNAMAALALCRAIGLPVSALLHGLRDYAGEPHRVELI
AAFDDIEFFDDSKGTNVGATVAALSGLSKHVVLIAGGDGKGQDFSPLAEPVAQYARAVIL
IGRDAPLIREALANTGVALIDAPTLEAAVQEAAAHAQPGDAVLLSPACASFDMFRNYEHR
AQVFHEAVVALAADRGVLL