Protein Info for Shew_1547 in Shewanella loihica PV-4
Annotation: phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to AROH_ECOL6: Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K01626, 3-deoxy-7-phosphoheptulonate synthase [EC: 2.5.1.54] (inferred from 100% identity to slo:Shew_1547)MetaCyc: 65% identical to 3-deoxy-7-phosphoheptulonate synthase, Trp-sensitive (Escherichia coli K-12 substr. MG1655)
3-deoxy-7-phosphoheptulonate synthase. [EC: 2.5.1.54]
Predicted SEED Role
"2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I alpha (EC 2.5.1.54)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.54)
MetaCyc Pathways
- superpathway of chorismate metabolism (51/59 steps found)
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- superpathway of L-phenylalanine biosynthesis (10/10 steps found)
- superpathway of L-tyrosine biosynthesis (10/10 steps found)
- chorismate biosynthesis I (7/7 steps found)
- 3-dehydroquinate biosynthesis I (2/2 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.54
Use Curated BLAST to search for 2.5.1.54
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QD66 at UniProt or InterPro
Protein Sequence (353 amino acids)
>Shew_1547 phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq) (Shewanella loihica PV-4) MTIKTDELRTTLLCKAISPAKLASEYPLTQDAADYLVQQRREVEAILTGEDKRLLVIIGP CSIHDTQAAIDYAKRLAVLHKELKDSLCILMRVYFEKPRTTVGWKGLISDPDLDGSFNAN KGLRLARHLLQEITELNLPIATEFLDMVNGQYIADLITWGAVGARTTESQIHREMASALS CPVGFKNGTDGNINIAIDAVRAAKEPHIFYSPDKDGAMAVYRTSGNPYGHIILRGGKQPN YHEADIQAAAQKLKDVGLSHRMVVDFSHGNSQKQHKRQLEVAKDIMTQVASGSTAIAGIM AESFIEEGNQKVVAGEPLIYGKSITDACLDWADSEKLLRDLAAASKARMAKLS